How Do Doctors Conduct a Mesothelioma Diagnosis?

A mesothelioma diagnosis is a type of cancer that’s often difficult to diagnose. It is most commonly caused by exposure to asbestos, a carcinogenic mineral widely used throughout the 20th century. Mesothelioma has a long latency period, meaning it can take decades after the initial asbestos exposure for the disease to develop.

Because mesothelioma is so rare and takes years to develop, it can take a long time to accurately diagnose the disease, which is usually done with the help of a radiologist. It can be a stressful and time-consuming process. If you show symptoms of mesothelioma, such as chest or abdominal pain or a persistent cough, contact your primary care doctor immediately, especially if you know or suspect that you have encountered asbestos in the past. Due to its aggressive nature, early detection can significantly improve a mesothelioma prognosis.


Video Transcript

“You have to have an adequate biopsy to make a diagnosis of mesothelioma. Considering that most of these are pleural, you would think the most obvious thing is involving the lung pleura, just by incidence, right? It’s much more common.

The first thing that comes to anyone’s mind; we just had an outside pathologist calling it adenocarcinoma. When you look at it and we had biopsies done in house and it doesn’t look like adenocarcinoma, they are cousins and they look very similar, but they have a completely different immune profile the way they mark and behave. That’s the first diagnostic consideration.

In mesothelioma when you get biopsies you are not sure if it is actually a mesothelial proliferation or if it’s malignant. So whether it’s benign or malignant, that becomes for the pathologist, the biggest challenge sometimes.”

– Vijayalakshmi Ananthanarayanan, MD
Surgical Pathologist | Loyola University Medical Center

Is Mesothelioma Difficult to Diagnose?

Generally, patients undergo a number of blood tests, imaging tests, and biopsies in addition to a thorough physical examination. This is all to locate where asbestos exposure has manifested in the body, which is usually near the lungs but can sometimes spread to the heart, stomach, and testicles.  Mesothelioma is often very difficult to diagnose because of its latency period and rarity. Luckily there are many options available to those seeking a diagnosis through a combination of these procedures and tests.

Imaging Tests for Mesothelioma Diagnosis

The mesothelioma diagnosis process generally begins with imaging tests, starting with the lowest resolution scans and working up if needed. This is crucial in locating mesothelioma.

This is an image of X-Ray results.

Does Mesothelioa Show on an X-Ray?

X-rays are low-resolution, low-radiation images that give doctors a two-dimensional glimpse into the body. Irregularities that may indicate the presence of mesotheliomas, such as fluid buildup, pleural thickening, pleural plaques, tumors, and irregularities in lung size, are usually visible with an X-ray. Abnormal scans may prompt doctors to move on to higher-resolution imaging tests. The average X-ray only takes around 15 minutes.

cartoon drawing of a blue CT scan machine

What does Mesothelioma look like on a CT Scan?

Computed tomography scans (also referred to as CT or CAT scans) combine rotating X-rays with computers to create multi-angled images of the body. They show if abnormalities are present and give doctors a deeper understanding of the extent and exact location of the abnormality, making them particularly helpful in diagnosing cancer. A CT scan lasts between 10 and 30 minutes, depending on the part of the body.

cartoon drawing of a blue PET scan machine showing the ribs

PET Scan for Mesothelioma Diagnosis

Positron emission tomography scans, or PET scans, use a small dosage of the radiotracer, a radioactive chemical, to produce three-dimensional images. PET scans can show changes in an organ or tissue at a cellular level, where most diseases begin. This technology allows doctors to determine the stage of mesothelioma based on how far it has spread throughout the patient’s body. Once patients are injected with the radiotracer, it takes about an hour to travel through the body and be absorbed by the affected organs or tissues. The actual scan takes an additional 30 to 60 minutes.

This is an image of MRI results.


MRIs (magnetic resonance images) use powerful magnetic fields and a computer rather than radiation to create high-resolution, three-dimensional images of affected areas in a patient’s body. They identify areas that may require a biopsy and are often used for staging mesothelioma. MRI test times range from 15 minutes to nearly an hour. Patients with claustrophobia (or a fear of enclosed spaces) may receive anti-anxiety medication, which requires a longer recovery.

This is an image of a mesomark, also known as a study of blood.

Blood Tests for Mesothelioma Diagnosis

Following imaging tests, blood tests are next in line when diagnosing mesothelioma. The most common blood tests doctors use to diagnose mesothelioma are listed below.

  • Cancer Antigen (CA 125) Cancer antigen 125 is a protein biomarker found in large concentrations of cancer cells. The CA 125 test measures how much protein is present in a patient’s blood, but it is not specific to mesothelioma and can be elevated in other tumor types. When factored in with stage and tumor location, a change in CA 125 levels can help determine a patient’s mesothelioma diagnosis and anticipate their response to systemic chemotherapy.
  • MesoMark® Gaining FDA approval in 2007, MesoMark® was the first blood test to aid in mesothelioma diagnosis or monitoring. MesoMark® works by measuring levels of soluble mesothelin-related proteins released by diseased cells, allowing doctors to assess the severity of mesothelioma present in the body. However, because sarcomatoid mesothelioma does not release these proteins, MesoMark® is unable to assist in the diagnosis or monitoring of this mesothelioma cell type and should be used in concurrence with other tests to ensure an accurate diagnosis.

This is an image of surgical tools.


A biopsy is the gold standard test to diagnose mesothelioma. It involves the removal of fluid or tissue in order to determine the presence or extent of the disease. In mesothelioma patients, biopsies allow doctors to determine whether cancer has spread and, if so, to what extent. Cytology reports are used to test samples retrieved from fluid biopsies for mesothelioma cells. Fluid biopsies are less invasive than tissue biopsies. However, because mesothelioma can mirror other conditions, a tissue biopsy is required to achieve a conclusive mesothelioma diagnosis and is usually necessary in order to pursue legal claims.

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The most common biopsies include needle biopsies, camera- or video-assisted biopsies, and open surgical biopsies.

This is an image of a needle biopsy.

Needle Biopsies

Sometimes called a closed biopsy, needle biopsies are the least invasive type of biopsy available for a mesothelioma diagnosis. They are generally outpatient procedures, meaning patients can come the same day.

  • Pleural Biopsy The most common needle biopsy, doctors perform pleural biopsies on patients experiencing fluid buildup between their lungs and chest (pleural effusion). An anesthetic is first injected to numb the area. Then, the doctor inserts a needle to collect a sample of the pleural fluid and tissue to check for the presence and extent of mesothelioma. The procedure usually lasts about 30 minutes to an hour.
  • Thoracentesis Also similar to a pleural biopsy, thoracentesis involves the draining of fluid in the pleural cavity. It is done for diagnostic purposes and to reduce patient pain and discomfort. The procedure is fast, usually around 15 minutes in total, depending on the amount of fluid removed.
  • Paracentesis Similar to a pleural biopsy, during paracentesis, a doctor inserts a needle into the patient’s peritoneal cavity to retrieve a sample of the ascitic fluid buildup in the abdomen. This procedure allows doctors to test for the presence of mesothelioma, as well as relieve patient discomfort. Depending on the level of fluid buildup, this procedure usually takes about 30 minutes.
  • Pericardiocentesis For patients experiencing fluid buildup around the heart sac (pericardial effusion), a pericardiocentesis procedure may be done to drain the fluid and prevent future buildup. Like other needle biopsies, pericardiocentesis can be helpful in the diagnostic process as well as alleviating patient pain. The procedure usually takes between 20 minutes to an hour.

This is an image of a camera biopsy tool.

Camera-Assisted Biopsies

Camera- or video-assisted biopsies involve a small camera, a computer, and surgical instruments. Generally, a doctor will perform a camera-assisted biopsy after a needle biopsy and before a surgical biopsy.

  • Thoracoscopy During this minimally invasive procedure, a surgeon makes a small incision to insert a viewing tube into the chest cavity. The doctor can capture a diagnostic sample and relieve the patient of some discomfort by draining excess fluid. The Thoracoscopy procedure ranges from 45 to 90 minutes.
  • Laparoscopy (Sometimes Called Peritoneoscopy) Similar to thoracoscopy, laparoscopy involves making a small incision that allows doctors to insert a viewing tube and a camera into the abdomen. Doctors are able to examine the affected abdominal organs and take diagnostic samples. This procedure usually lasts around 75 minutes.
  • Mediastinoscopy This camera-assisted biopsy procedure allows doctors to examine the lymph nodes and see if the mesothelioma has spread. It generally takes about 60 to 90 minutes.

This is an image of surgical tools.

Surgical Biopsies

A surgeon will perform a surgical biopsy when less invasive biopsies fail to provide definitive results. They are conducted under general anesthesia.

  • Thoracotomy When a tumor is detected in the chest, a thoracotomy may be performed. During this surgical procedure, the surgeon makes an incision in the chest to take a tissue biopsy. When possible, the surgeon will also remove as much of the tumor as possible during this procedure.
  • Laparotomy When a tumor is detected in the abdomen, a laparotomy may be performed. During this surgical procedure, the surgeon makes an incision in the abdomen to take a tissue biopsy. When possible, the surgeon will also remove as much of the tumor as possible during this procedure and consider what is called cytoreductive surgery with intraperitoneal heated chemotherapy. The latter is not for diagnosis but for treating peritoneal mesothelioma.

What to do After a Mesothelioma Diagnosis?

If you have seen a specialist and been diagnosed with mesothelioma or asbestosis, you may now want to be connected to a doctor who has experience in the field of treating and diagnosing mesothelioma. It can be a worrisome time, so let the professionals at Mesothelioma Hub help you through the process, especially when seeking a second opinion.

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